puromycin In A Sentence

We found 18 'puromycin' sentence examples to help you understand how to use puromycin in a sentence.

• When the ribosome reaches the 3' end of the template, the fused puromycin will enter the A site of the ribosome . b.
• These include anisomycin, cycloheximide, chloramphenicol, tetracycline, streptomycin, erythromycin, and puromycin.
• This enzyme participates in puromycin biosynthesis.
• "' puromycin "'is an antibiotic that is a protein synthesis inhibitor by inhibiting translation.
• Lethal concentrations of puromycin are much higher for strains of " Saccharomyces cerevisiae " than mammalian cell lines.
• This can be covalent, such as mRNA display where the mRNA gene is linked to the protein at the end of translation by puromycin.
• Also of note, puromycin is critical in mRNA display.
• Objective: To investigate the effects of Chaihuang-Yishen Formula Extract (CYE)on urinary protein and the function of fibrinolysis of rats with nephrosis induced by puromycin aminonucleoside (PAN).
• Once the mRNA library is generated, it will be Urea-PAGE purified and ligated using T4 DNA ligase to the DNA spacer linker containing puromycin at the 3' end.
• Puromycin is non-selective, inhibiting RNA blocking ribosomal translation.
• The present study is the first to report on the usefulness of puromycin for production of enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer.
• Compared to the cleavable ester bond in a tyrosyl-tRNA, puromycin has a non-hydrolysable amide bond.
• The antibiotic-resistant transfected cells were selected and enriched by applying puromycin in culture medium.
• All mRNA templates used for mRNA display technology have puromycin at their 3' end.
• Puromycin aminonucleoside is the key intermediate in the synthesis of semi-synthetic analogues of puromycin.
• The ligated mRNA-DNA-puromycin library is translated in Red Nova Lysate ( Novagen ) or " E . Coli".
• Puromycin is soluble in water 50mg/ml as colorless solution at 10 mg/ml.
• Puromycin is an aminonucleoside antibiotic, derived from the " Streptomyces alboniger " bacterium, that causes premature chain termination during translation taking place in the ribosome.